
Dayalbagh Educational Institute (Deemed to be University)
Mob:+91 9410421149
Courses Taught:
- Microbial Technology Food Quality Systems, Assurance & Management
- Fundamentals of Microbiology & Biochemistry
- Food Safety Standards & Regulatory Affairs
- National & Global Food Security
- Quality Control of Food & Dairy products
- Quality Management Systems
- Industrial Microbiology & Food Biotechnology
- Food & Personal Hygiene
- Principles of Food Processing
Incharge, Food & Dairy Testing Lab(FDTL), Department of Botany, Dairy Campus, Dayalbagh Educational Institute. Have established the Microbiological wing, Wet Chemistry and Instrumentation wing of the FDTL
Recent Highlights
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Empaneled member National Scientific Committee of Sampling & Analysis, Science & Standards Division, Food Safety & Standards Authority of India (FSSAI)
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Empaneled member Microbiology Sub-Committee of Sampling & Analysis, Science & Standards Division, Food Safety & Standards Authority of India (FSSAI)
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National Trainer on Food Safety & Hygiene, Advanced Catering (Schedule-4), Food Safety Training & Certification (FOSTAC), Food Safety & Standards Authority of India (FSSAI)
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Training in Commisioning and Operation of Rapid Microbiological Enumeration and Detection System by Biomerieux – VIDAS & TEMPO, a Enzyme Immuno Assay based Rapid Detection System
- Training in Commisioning and Operation of ‘Extenso’(Unisensor), a Enzyme Immuno Assay based Rapid Detection System for Antibiotic Residue and Mycotoxins in Milk and Milk Products
- Training in handling HeLa cells at National Institute of Material Science, Tsukuba, Japan
Professional Experience:
Research & Teaching::
- Principal Investigator of a research project under the Young Scientist Scheme of the Science & Engineering Research Board (SERB), Department of Science & Technology, Govt. of India. – 3 years
- Head, Biotechnology & Dean, Training & Development (Dual Role) at the Uttam Group of Institutions (UGI), Runakta, Agra, UP, INDIA. – 1 year
Industry (2000 to 2008):
- Unit Manager (Quality & Purchase), at Bikanervala Food Pvt Ltd (BFPL), Agra Region.
- Consultant, Training & Audits, Sinar Jernih India Pvt Ltd –
- Head (Asst. Manager), Quality Assurance, TajSATS Air Catering Ltd. New Delhi.
- In-charge Operations, Multilabs & Microbiology Section (Dual Role), SGS India Pvt Ltd., Multilabs Division (3rd party testing facility), Gurgaon
- Quality Assurance Executive & Regional Microbiologist, TajGVK Hotels, Hyderebad / TajSATS Air Catering Ltd., Delhi. Consecutive posting from Hyderabad to Delhi.
Research Profile
Research Interest
● Psychobiotics, the Gut-Brain Axis & the Gut Microbiome
● Probiotics & Human Health
● Molecular Diagnostics & Biosensing
● Microbial Ecology & Sociobiology of microbes.
● Food safety in relation to food security & Agroecology
Current Projects
Socio-economic Empowerment of the Tribal People in the Rajaborari Estate, Madhya Pradesh through Scientific and Technological Interventions – DST/SEED Scheme – 2.5 Crores
Projects Completed
- A study for identification of global expression of specific genes during and for biofilm formation in Bacillus subtilis in comparison to Bacillus mojavensis. (Ref.No:-SERB/LS-200/2013) http://serb.gov.in/pdfs/Archive/FTYS-life-sciences-IV-meeting-result.pdf
- Development of Low-cost compact Milk Adulteration Test Kit – ‘KEMAT’ (Kit for Easy Milk Adulteration Testing) https://www.youtube.com/watch?v=WYePWB8PkIk&list=PLabdhsLUjJQFo-x4SKwMzGTpYyPj4N2eo&index=59&t=0s
- Development of Mobile Milk Testing Unit – on the ‘goMATA’ (on the go Milk Adulteration Testing Assistance)
Research Collaborations:
Dr.Evangelyn Alocijla, Nano-Biosensor Lab, College of Engineering, Michigan State University, Lansing, Michigan USA. https://www.egr.msu.edu/alocilja/GARD-location/global-alliance-rapid-diagnostics-india
Presentations & Invited Talks
1. Possible applications of Nano-silver coatings in the food processing industry to control biofilms at the International Advanced Research Centre for Powder Metallurgy and New Materials (ARCI), Hyderabad, India. dated the 2nd February 2010.
2. Listeria monocytogenes J0161 responding to competition, as stress, in broth and biofilms with reference to gene expression pattern, 1st to 4th July 2012, the Annual Scientific Meeting and Exhibiton (ASM 2012) at the Brisbane Convention and Exhibition Centre, Brisbane, Australia.
3. Microarray Technology & Transcriptomics at Specturm Institute of Science & Technology, Colombo,SriLanka., dated the 11th June 2015.
4. Mood Swings, Gut Microbes & Food Traditions, 5th to 11th June 2017, The Science of Consciousness (TSC2017) at San Diego, USA.
5. A Subject of Evolution - Homo spiritualis, 2nd to 7th April 2018, The Science of Consciousness (TSC2018) at Tucson, USA
6. Panelist, Indian International Food Safety Conclave, 7th to 8th November 2019 at Indore, India.
7. Invited Speaker, Student Orientation Program, Department of Microbiology, D G Vaishnav College, Chennai, India, 2020.
8. Invited Speaker, Global Alliance on Rapid Diagnostics Symposium, Michigan State University, USA, 2020.
9. Invited Speaker, Recent Advances in Biochemical and Material Sciences, Chennai, INDIA, 2020.
10. Street Food Vendor Development towards ‘Unnat Bharat Abhiyan’, 2nd December 2019, DEI.
11. PRPs to Food Safety, a tripartite discussion, 23rd September 2020, organized by FSSAI.
12. Invited Speaker, Induction Program, 5th November 2020, P.G. and Research Department of Microbiology, Dwaraka Doss Goverdhan Doss Vaishnav College, Chennai.
13. Invited Speaker, Transcriptomics for Understanding Life Functions, 25th November 2020, International Conference on Recent Advances in Biochemical & Material Sciences 2020
1. IInd Annual Conference of Indian Association of Applied Microbiologists (Chennai, 2002)
2. Workshop on Food Microbiology, Science & Application (Hyderabad, 2004)
3. Annual Scientific Meeting of Australian Society for Microbiology (Brisbane, 2012)
4. Antimicrobial Workshop – Antimicrobial and Antifungal Resistance : Susceptible Testing – Supplementary and Molecular testing for Multi-Drug Resistance. (Brisbane, 2012).
5. God & Genetics (Short Course), Faraday Institite of Science & Religion, University of Cambridge (Cambridge, UK, 2012)
6. The Science of Consciousness (TSC2017)(San Diego, USA, 2017)
7. The Science of Consciousness (TSC2018)(Tucson, USA, 2018)
8. Indian International Food Safety Conclave (Indore, India, 2019)
9. Golobal Alliance on Rapid Diagnostics Symposium (Michigan State University, 2019)
10. Recent Advances in Biochemical and Material Sciences, (Chennai, INDIA, 2020.)
1. Gene Expression Omnibus (GEO), NCBI Submission – 08 Microarray data of L.monocytogenes J0161 – Accession Number – GSE27936.
2. SRA on Transcriptome analysis, NCBI submission – 01 bio-project, 06 bio-samples of Bacillus subtilis & Bacillus licheniformis. Bio-project ID-PRJNA336278
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Citation profile (as in February 2022)

Publication List
1. Tirumalai, P. S., & Prakash, S. (2011). Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and co-culture broths. African J Microbiol Res, 5, 5188-5193. doi:10.5897/AJMR11.929
2. Tirumalai, P. S., & Prakash, S. (2012). Time-dependant gene expression pattern of Listeria monocytogenes J0161 in biofilms. Advances in Genomics & Genetics, 2, 1-18. doi: 10.2157/AGG.S26335
3. Tirumalai, P. S., & Prakash, S. (2012). Antibiotic resistance in co-culture biofilm of Listeria monocytogenes J0161. American. J. Microbiol, 3, 7-17.
4. Tirumalai, P. S., & Prakash, S. (2012). Expression of virulence genes by Listeria monocytogenes J0161 in natural environment. Brazilian J of Microbiol, 43(2), 834-843. doi: 10.1590/S1517- 83822012000200050.
5. Sharma, R., Daya, K.S, & Tirumalai, P. S. (2012). Light assisted in-vivo microwave sensing for electrical characterization of prokaryotes. Biophysical Reviews and Letters, 7 (03n04), 219-227. doi:10.1142/S1793048012500105.
6. Tirumalai, P. S. (2015). Metabolic gene expression shift by Listeria monocytogenes in coculture biofilms. Canadian J of Microbiol. 61(5): 327-334, 10.1139/cjm-2014-0704.
7. Zeeshan.,Daya, K. S., Tirumalai, P. S., & Alocilja, E. (2018). Impedance and Magnetohydrodynamic Measurements for Label Free Detection and Differentiation of E.coli and S.aureus Using Magnetic Nanoparticles. IEEE transactions on nanobioscience, 17(4), 443-448.
8. Prakash, S., & Tirumalai, P. S. (2012). Microbial Systems Biology: A Road Less Travelled. American J of Microbiol, 3(1), 1-6.
9. Tirumalai, P.S, and Bagchi, A.K. (2013) Magnum opus: Clustered regularly interspaced short palindromic repeats biology and prokaryotic gene silencing. American J of Immunol .9(4),110. doi:10.3844/ajisp.2013.110.115.
10. Kumar, V., Tirumalai, P. S., Singh, A., Bhatnagar, A. K., & Shrivastava, J. N. Natural Compounds from Algae and Spirulina platensis & its Antimicrobial Activity. Indo Global J of Pharm Sc. 3(3): 212-223.
11. Tirumalai, P. S. (2013). Listeriosis and Listeria monocytogenes in India.Wudpecker J Food Technol, 1(6), 98-103.
12. Tirumalai, P. S. (2015) Understanding social interactions of microbes using transcriptomics : A Codicil. J Microbiol, Biotech & Food Sci. 4 (6) 491-495. doi:10.15414/jmbfs.2015.4.6.491-495.
1. Oberoi, Sudhi, K. Soami Daya, and Prem Saran Tirumalai. "Microwave sensor for detection of E. coli in water." Sensing Technology (ICST), 2012 Sixth International Conference on Sensing Technology. IEEE, 2012.
2. Daya, K. S., Tirumalai, P. S., Baum, M., & Adelung, R. (2019, March). Understanding the Interaction of Escherichia coli with ZnO Tetrapods at Microwave Frequencies. In 2019 URSI Asia-Pacific Radio Science Conference (AP-RASC) (pp. 1-4). IEEE.
3. Sharma, Shreya., and Prem Saran Tirumalai. A Pilot Study on the Application of Functionalized Magnetic Nano Particles For Detection and Extraction of Pathogens In Beverages. In AMI-2019 60th Annual Conference of Association of Microbiologists of India.
4. Siddiqui, Talha., and Prem Saran Tirumalai.Competitive Inhibition of Listeria monocytogenes by Lactic Acid Bacteria. In AMI-2019 60th Annual Conference of Association of Microbiologists of India.
Masters Dissertations Completed
- A Pilot Study on the Application of Functionalized Magnetic Nano Particles For Detection and Extraction of Pathogens In Beverages
- Efficacy of Pseudomonas fluorescens and Bacillus subtilis as pure and mixed culture for Bio-remediation.
- Investigative study on the Formulation Framework of Ready-to-Eat (RTE) Curry with Reference to Palak Paneer
- Understanding the development of Ready-to-Eat Kadhi Chawal from procurement to distribution.
- Study on complete processing of dehydrated RTE Samosa
- Competitive Inhibition of Listeria monocytogenes by Lactic Acid Bacteria
Research work under supervision
- Isolation and efficacy study of bacteriophages from drains(effluents) as bio-control agents, specific to E.coli & Salmonella Spp for possible application in the beverage industry.
- Survey to detect pathogen(E.coli & Salmonella Spp) contamination in local/rural supply of bottled (potable) water in compliance to the Indian Standard (IS:14543)
- Effect of food additives on the strains of Lactobacillus Spp & Bifidobacterium Spp
- Efficacy of Lactobacillus Spp & Bifidobacterium Spp in biological detoxification of bacterial exotoxins
- Prospecting the probable application of Lactobacillus Spp & Bifidobacterium Spp in cancer therapy, with special reference to Colo-rectal cancer, via carcinogen degradation.
- Probing for the possible role of (vegan) diet in mood modulation and meditation
Media Publications
- YouTube Channel – ‘Open to All’ https://studio.youtube.com/channel/UC-bfmuYAycDOJ8ZV9zG2Z0w
- Mood-Swings & Gur Microbes – Editorial, The Speaking Tree, Times of India https://www.speakingtree.in/article/mood-swings-gut-microbes
- A Lesson Learnt - Editorial, The Speaking Tree, Times of India https://www.speakingtree.in/article/a-lesson-learnt
- My Daughter My Teacher – Editorial, The Speaking Tree, Times of India https://www.speakingtree.in/article/my-daughter-my-teacher
Consultancy & Startups
- Honorary consultant, (Food Safety & Hygiene, Lab Design Turn Key projects) with The Rethink Hospitality Pvt Ltd. https://rethinkhospitality.business.site/
- Director, M/s. SolvoDominum, a startup company of the Dayalbagh Educational Institute, offering innovative technical solutions to the industry. https://www.zaubacorp.com/company/SOLVODOMINUM-PRIVATE-LIMITED/U74999UP2020PTC139668